Performance

Traditionally, pooled microsomes have consisted of a range of individual donors in order to attain average enzyme activities. These pooled microsomes have been used to answer various metabolic questions. However, they have not always performed sufficiently for certain applications, such as inhibition studies. The approach—which averages all types of enzyme activity—may not provide the complete insight into compound performance that you seek.

In ADME-Tox research, different experiments can benefit from different enzyme activities. That’s why we’ve changed how we formulate microsomes to give you a choice. With our unique purpose-pooled InVitroCYPs, you can select enzyme activity levels in target ranges across nine key isoforms.

For inhibition studies, InVitroCYP H-class microsomes provide higher activities that allow you to determine inhibition with confidence. In fact, InVitroCYPs are the only microsomes on the market with substrate data reported at both Km and Vmax concentrations, providing you with maximum visibility for predictive performance and quality.

To view our microsome products, click here.

Quality & The Celsis 3-C Process

To guarantee InVitroCYP’s quality, unsurpassed lot-to-lot consistency, and the targeted activity levels you need in your research, Celsis IVT developed an exclusive 3-C process for microsomes:

1. Characterize all tissue, at Km and Vmax levels, before blending.

2. Combine using a proprietary algorithm to ensure optimum enzyme activity for the different product classes across nine key CYPs.

3. Certify that the final products meet target specifications, including total P450 levels, through Celsis’ independent Quality Assurance/Quality Control department.